| Registro completo |
| Provedor de dados: |
Biol. Res.
|
| País: |
Chile
|
| Título: |
Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
|
| Autores: |
Guo,Jian-Ru
Chen,Qian-Qian
Lam,Christopher Wai-Kei
Zhang,Wei
|
| Data: |
2015-01-01
|
| Ano: |
2015
|
| Palavras-chave: |
Karanjin
Cell cycle
Apoptosis
Cancer therapy
|
| Resumo: |
BACKGROUND: We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). RESULTS: MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin exhibited significantly increased apoptotic rates, especially in the percentage of late apoptosis cells. CONCLUSION: Karanjin can induce cancer cell death through cell cycle arrest and enhance apoptosis. This compound may be effective clinically for cancer pharmacotherapy.
|
| Tipo: |
Journal article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100040
|
| Editor: |
Sociedad de Biología de Chile
|
| Formato: |
text/html
|
| Fonte: |
Biological Research v.48 2015
|
|
